Extended Data Fig. 10: Measurement of the activity of GRAND editing at predicted off-target sites and usage of different transfection reagents to deliver GRAND editing in HEK293T cells.
From: Efficient targeted insertion of large DNA fragments without DNA donors
a, The predicted off-target sites of single pegRNA were selected using Cas-OFFinder, and amplicon sequencing was performed. b, To determine efficiencies of 150 bp insertion using four transfection reagents by real-time qPCR. Mean ± s.d. of n = 3 independent biological replicates.
