Extended Data Fig. 8: Whole-brain, neuron-level 3D imaging in larval zebrafish in vivo.
From: DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy
High-resolution images are recorded in steps of 8 μm with an exposure time of 8 ms. A volume of 500 μm * 300 μm * 200 μm, containing the entire brain, is recorded once every 0.3 s. The recording of two different zebrafish lines is shown in (a)-(c) and (d)-(f). (a) and (d) show the max intensity projection of the 3D volumes. (b) and (e) show regions of interest from a single slice of the 3D stack, indicated by the write rectangles in (a) and (d). (c) and (f) give representative fluorescence signal of five different neurons over time.
