Extended Data Fig. 8: Molecular spike internal barcode-based expression standard curves.
From: Molecular spikes: a gold standard for single-cell RNA counting
For each of the 11 distinct spike-in sequences, we show the total number of unique spike-in molecules per barcode (y-axis; log2 transformed axis) when sequenced to saturation. On the x-axis, we denote the expected expression rank of each barcode. Linear regression shown as line and 95% confidence interval as gray shaded area.
