Extended Data Fig. 1: Quality control of 5ˈ molecular spike-in.
From: Molecular spikes: a gold standard for single-cell RNA counting
(a) Sequence logo of the 18 random spUMI bases derived from all reads in the Smart-seq3 dataset. At each position, the frequency of all 4 bases is visualized by the size of the DNA letter. (b) Minimal distance of uncorrected spUMIs to the closest spUMI sequence for all pairwise within-cell comparisons and pairwise comparisons of spUMIs to 1000 randomly samples spUMI sequences across cells (total 2,233,878 comparisons). (c) Cumulative number of molecular spikes (n = 885,925) sorted by their occurrence over cells (n = 340). Dashed line indicates the chosen QC cutoff at 4 cells.
