Extended Data Fig. 2: Expression levels in HEK293FT cells.
From: Molecular spikes: a gold standard for single-cell RNA counting
(a,b) Quality of Smart-seq3 libraries (n = 111 cells) after filtering. Shown are the number of detected (a) genes and (b) molecules per HEK293FT cell. Boxplots show the median, first and third quartiles as a box, and the whiskers indicate the most extreme datapoint within 1.5 lengths of the box. (c) Histogram showing the mean UMI count per cell for all genes expressed in at least 50% of cells (n = 10,198 genes). (d) Schematic overview of the counting-critical steps in the Smart-seq3 protocol. (e) Schematic overview of the evaluated protocol variations for the SCRB-seq library preparation.
