Extended Data Fig. 3: Counting difference in Smart-seq3 protocol variations.
From: Molecular spikes: a gold standard for single-cell RNA counting
(a,b) For variations of the Smart-seq3 protocol, molecular spikes were sampled at varying mean expression levels. Colored lines indicate the mean counting difference in (a) absolute numbers or (b) relative to the mean and shaded by the standard deviation for library preparation conditions 0.1 µM FWD (n = 48 cells), TSO + 0.1 µM FWD (n = 48 cells), TSO + 0.5 µM FWD (n = 39 cells) and TSO + 1.0 µM FWD (n = 45 cells).
