Extended Data Fig. 8: NODO imaging quality versus imaging depth in a PEGASOS-cleared mouse brain.
From: A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues
(a) A single ROI was acquired from a PEGASOS-cleared mouse brain. (b) xz view of the ~5 mm deep ROI, demonstrating near-consistent image quality versus depth. (c-g) xy views for ROIs at various depths are shown. The high-magnification insets show fine sub-nuclear details and demonstrate that there is minimal degradation in image quality as a function of depth. Scale bar lengths are as follows: (b) 100 µm, (c-g) 10 µm. The imaging data in (b-g) was acquired from in a single experiment, which was not repeated.
