Extended Data Fig. 9: NODO imaging quality versus imaging depth in an ECi-cleared mouse brain.
From: A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues
(a) Multiple regions of interest (ROIs) were acquired from an ECi-cleared mouse brain at various imaging depths (z = 0–5 mm). (b-f) xy views for ROIs at various depths are shown. The corresponding xz views of each ROI are shown in (g-k). The high-magnification insets show fine sub-nuclear details and demonstrate that there is minimal degradation in image quality as a function of depth. All scalebars denote 10 µm. The imaging data in (b-k) was acquired from in a single experiment, which was not repeated.
