Extended Data Fig. 3: Assessment of 3D cell proliferation assays with iDISCO.
From: A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues
(a) Screening of an intact mouse prostate cleared using iDISCO, labeled with TOPRO3 Iodide (nuclear) and an EduClick cell-proliferation marker. A higher-resolution region of interest focused on a prostate gland is shown in (b). An additional zoomed-in view showing the ability to resolve individual proliferating nuclei (c). Scale bars lengths are as follows: (a) 1 mm, (b) 200 μm, and (c) 100 μm. The imaging data in (a-c) was acquired from in a single experiment, which was not repeated.
