Extended Data Fig. 5: Imaging of immunostained and endogenously fluorescent CUBIC-cleared mouse brains. | Nature Methods

Extended Data Fig. 5: Imaging of immunostained and endogenously fluorescent CUBIC-cleared mouse brains.

From: A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues

Extended Data Fig. 5

Hybrid OTLS imaging of whole mouse brains. (a-c) with endogenously preserved GCaMP6s fluorescence, and immunostained with (d) anti-ChAT antibody + SYTOX-G or (e) anti-Parvalbumin (PV) antibody + SYTOX-G by CUBIC-HistoVision. Scale bar lengths are as follows: (a-b) 1 mm, (c) 10 μm, (d-e) 2 mm. The imaging data in (a-e) was acquired from in a single experiment, which was not repeated.

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