Fig. 4: Surface passivation. | Nature Methods

Fig. 4: Surface passivation.

From: Label-free nanofluidic scattering microscopy of size and mass of single diffusing molecules and nanoparticles

Fig. 4

a, Schematic of SLB formation. LUVs flow through the nanochannel, adsorb on the nanochannel wall, rupture and create patches of lipids that eventually connect and create a homogenous layer. b, Kymograph capturing the SLB formation in Channel V, as manifested by decrease in scattering intensity. c, Schematic of a biomolecule diffusing inside a nanochannel coated with an SLB. d,e, Inferred molecular weight (d) and Rs (e) of the positively charged protein aldolase measured in Channel VI and analyzed by ML. The arrows indicate the nominal values (MW = 158 kDa, RS = 4.6 nm (ref. 32)).

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