Extended Data Fig. 9: Scatter plot of the rosette streak volumes vs the average Sox2.
From: Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification
Expression levels per nucleus were compared for each ‘O-shape’ organoid. Each individual point represents one organoid (N=96 organoids, N=415 streaks). The populations were classified in 4 groups using gating based on the Sox2 intensity threshold established in Fig. 4b and Methods. Images display examples of organoids in each of the 4 groups. White lines represent the Deep learning-based segmentation of the streak contours. Box plots represent the distributions of the number of nuclei and mitotic indexes for the −/− and +/+ gated populations. ** : p-value = 0.006; * : p-value =0.05 were obtained by unpaired Student T-test. Grey values (687 and 2,0) represent the mean values for the entire ‘O-shape’ population of organoids. The graph displays the mean (red line), the Standard deviation (box) and the min-max (whiskers).
