Extended Data Fig. 6: AAV-MGs effectively transduce neurons and astrocytes in vivo but do not efficiently transduce cultured microglia.
From: Directed evolution of adeno-associated virus for efficient gene delivery to microglia
a, Representative images showing the mScarlet expression patterns in the striatum of C57BL/6 N mice injected with a virus mixture of AAV-Ef1a-Cre and AAV-MG1.1-SFFV-DIO-mScarlet or a virus mixture of AAV-Ef1a-Cre and AAV-MG1.2-SFFV-DIO-mScarlet. Yellow: mScarlet, blue: DAPI. Scale bars, 500 µm (slide scanning) and 100 µm (confocal images). b, Representative images showing the colocalization of mScarlet and NeuN+/GFAP+ immunosignals in the striatum of C57BL/6 N mice injected with a virus mixture of AAV-Ef1a-Cre and AAV-MG1.1-SFFV-DIO-mScarlet or a virus mixture of AAV-Ef1a-Cre and AAV-MG1.2-SFFV-DIO-mScarlet. Scale bars, 50 µm. c, Representative fluorescent and bright-field images of cultured mouse microglia transduced with mScarlet reporter rAAVs packaged using AAV-MG1.1 or AAV-MG1.2. Scale bars, 200 µm.
