Extended Data Fig. 9: AAV-MG1.2 enables in vivo two-photon imaging of microglia extracellular ATP changes following acute laser ablation.
From: Directed evolution of adeno-associated virus for efficient gene delivery to microglia
a, Representative images showing the colocalization of jGCaMP8s (HA-tag immunosignals) and Iba+ immunosignals in the primary somatosensory (S1) cortex of Cx3cr1CreER mice injected with AAV-MG1.2-SFFV-DIO-jGCaMP8s. Scale bar, 50 µm. b, Representative images showing the colocalization of GRABATP1.0 (HA-tag immunosignals) and Iba+ immunosignals in the S1 cortex of Cx3cr1CreER mice injected with AAV-MG1.2-SFFV-DIO-GRABATP1.0. Scale bar, 50 µm. c, Quantification of GRABATP1.0 fluorescence signals at microglial somata in the control mice (n = 10 cells; black) or in the mice that received laser ablation (n = 16 cells; red) (two-way ANOVA; P values as listed in the figure). Data are presented as mean ± s.e.m. d, Image showing GRABATP1.0 expression in microglia and heatmaps showing the GRABATP1.0 fluorescence signals at 10, 20, and 40 min in the imaging session. For the laser ablation group, the laser was applied at the center of field of view at the beginning (0 min) of the imaging session. Scale bar, 100 µm.
