Extended Data Fig. 10: In vivo microglia-specific gene knockout mediated by AAV-MGs.
From: Directed evolution of adeno-associated virus for efficient gene delivery to microglia
a, Representative images of serial coronal sections of the striatum from a Cx3cr1CreER:Rosa26-LSL-Cas9-GFP mouse injected with AAV-MG1.1-sgRNA-LacZ or AAV-MG1.1-sgRNA-Tmem119. The brain sections were immunostained against Tmem119. Scale bar, 500 µm. b, Representative images of serial coronal sections of the striatum from a Cx3cr1CreER:Rosa26-LSL-Cas9-GFP mouse injected with AAV-MG1.2-sgRNA-LacZ or AAV-MG1.2-sgRNA-Cd68. The brain sections were immunostained against Cd68. Scale bar, 500 µm. c, Representative images of the S1 cortex of Cx3cr1GFP mice injected with AAV-MG1.2-CMV-SaCas9 or AAV-MG1.2-CMV-SaCas9-U6-sgRNA-P2ry12. The brain sections were immunostained against P2ry12. Scale bar, 100 µm. d, Quantification of the percentage of P2ry12-positive pixels in a 1 × 1 mm2 region in the S1 cortex of Cx3cr1GFP mice injected with AAV-MG1.2-CMV-SaCas9 (n = 2) or AAV-MG1.2-CMV-SaCas9-sgRNA-P2ry12 (n = 3) (two-way ANOVA; P values as listed in the figure). Data are presented as mean ± s.e.m.
