Extended Data Fig. 3: Schematic of in vivo selection of AAV-cMG.WPP variants library by the Cre recombination-based AAV targeted evolution (CREATE) strategy.
From: Directed evolution of adeno-associated virus for efficient gene delivery to microglia
The AAV-cMG.WPP variants library was injected into the striatum and the midbrain of Cx3cr1CreER mice, which selectively express Cre recombinase in microglia. For those variants that successfully transduced microglia, Cre flipped the double-floxed inverse open reading frame (DIO) on their AAV genomes, allowing the selective amplification and recovery of the heptamer insertion sequences using a pair of primers.
