Extended Data Fig. 8: Additional excerpted views from a ChAT SPEAR-stained SHIELD-protected whole mouse brain.
From: Antibody stabilization for thermally accelerated deep immunostaining
a, Enlarged view of the whole mouse brain shown in Fig. 4g. b, Volume-rendered coronal plane view of the striatum (Str) and nucleus basalis of Meynert (NbM), demonstrating cholinergic fibers in the anterior commissure, basal forebrain and striatum. c, Cholinergic fibers (arrowheads) in layer 1 of inferior frontal cortex. Inset shows an enlarged view. d, Cholinergic tractographies grouped according to anatomical landmarks. Selected anatomical regions were manually segmented and ChAT-positive fibers that traverse the segmented region are displayed individually (upper panels) or in combination (lower panel). Fibers are color coded according to their middle segment directionality. Experiment performed once.
