Extended Data Fig. 9: Additional excerpted views from a PV SPEARpy-stained whole mouse brain processed with the iDISCO protocol.
From: Antibody stabilization for thermally accelerated deep immunostaining
a, Enlarged whole-brain 3D-rendered view. b, Another view of the cerebellar hemisphere shown in Fig. 4r, overlaid with identified PV-positive cells. Inset shows a further excerpted view demonstrating accurate localization of spots. c, Sagittal slice 3D rendering as indicated by the yellow boxed area in a. Individual PV-positive cells are visible from this volume rendering. d, Block 3D rendering as indicated by the green boxed area in a. Enlarged views from different brain regions from different z-slices were excerpted and displayed in the right panels, with further enlarged views for red boxed areas showing cells in insets. Experiment performed once.
