Extended Data Fig. 4: Pro-T/iPSC-TEC thymus organoids support progression of T cell development in vitro. | Nature Methods

Extended Data Fig. 4: Pro-T/iPSC-TEC thymus organoids support progression of T cell development in vitro.

From: De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids

Extended Data Fig. 4

a. FCM analysis of CD4 and CD8 expression in cells of Pro-T/iPSC-TEC thymus organoids (lower panels) after one week of in vitro culture. Upper panels, cells dissociated from one week cultured decellularized scaffolds seeded with Pro-T cells alone (Pro-T scaffolds) as controls. Shown are representative FCM graphs from at least three independent experiments with similar results. b. FCM analysis of thymic output from Pro-T/iPSC-TEC thymus organoids (lower panels) and Pro-T scaffold controls (upper panels). hCD45+ cells were examined for key surface markers of T cell development (CD1a + CD3 + , left columns), TCRab (middle columns), and CD4 and CD8 (right columns). Shown are representative graphs of at least two independent experiments for the Pro-T control output and at least five independent experiments for Pro-T/iPSC-TEC thymus organoid output with similar results.

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