Extended Data Fig. 7: Multiple hematopoietic lineage development in hu.Thor mice after 4-weeks of iPSC-TEC thymus transplantation. | Nature Methods

Extended Data Fig. 7: Multiple hematopoietic lineage development in hu.Thor mice after 4-weeks of iPSC-TEC thymus transplantation.

From: De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids

Extended Data Fig. 7

Gating strategy for characterization of hu.Thor splenocytes. Singlets were then gated on hCD45 versus mCD45, and only hCD45+ cells were used as the entry gate into lineage study. Staining was completed for T cells (CD4 and CD8, top right panel), B cells and dendritic cells (CD20 and CD11c, second from top right panel), myeloid and NK cells (CD33 and CD56, third from the top right panel), and monocyte cells (CD14, bottom panel). Shown are representative of three independent experiments with similar results.

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