Extended Data Fig. 9: CytoStim-mediated IL-2 and IFNg production in hu.Thor CD3 + T-cells.
Splenocytes and bone marrow cells were harvested and mixed at 1:1 ratio from hu.Thor (n = 4) and hu.PBMC (n = 3), and stimulated with CytoStim (a and c, lower panels) for 6 hours, and intracellularly stained for IL-2 (a and b) and IFNg (c and d). a and c. FCM analysis of IL2- (a) and IFNg- (c) producing cells in the hCD45 + CD3 + T cell populations. Shown are representative FCM graphs from three independent experiments with similar results. b and d. Percentages of IL2 + (b) and IFNg + (d) hCD3 + T cells before (blue dots) and after (red dots) CytoStim stimulation. Data were analyzed with paired, two-tailed t-test. p < 0.05 is considered as significance.
