Extended Data Fig. 10: Generation of major human immunoglobulin classes in hu.Thor mice. | Nature Methods

Extended Data Fig. 10: Generation of major human immunoglobulin classes in hu.Thor mice.

From: De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids

Extended Data Fig. 10

Sera were harvested from hu.Thor mice (n = 15) at 16-18 weeks post-transplantation. Isotypes of human immunoglobulin classes were quantified with Luminex isotyping kit. Upper rows show the primary classes of immunoglobulin. Lower rows show the IgG subclasses. Sera from hu.SRC mice (n = 8) at similar post-transplant ages were used as controls. Sera from untreated NSG mice (n = 8) were used as negative control to show the background of the assay. Data are shown as dot plots with mean (bar). Statistical analysis was performed between hu.SRC and hu.Thor samples with a two-tailed Mann-Whitney test. p < 0.05 is considered as significance.

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