Extended Data Fig. 2: Immunofluorescence analysis of human FOXN1 expression in 3D iPSC-TECs. | Nature Methods

Extended Data Fig. 2: Immunofluorescence analysis of human FOXN1 expression in 3D iPSC-TECs.

From: De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids

Extended Data Fig. 2

Immunofluorescent images of 3D iPSC-TEC aggregates stained with antibodies against human FOXN1 (red) and counterstained with Hoechst 33342 for nucleus (blue). Primary human thymic epithelial cells and lymph node fibroblasts were used as positive and negative controls, respectively. White scale bar, 25um. Shown are representative images from three independent iPSC-TEC differentiations with similar results.

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