Extended Data Fig. 3: etSTED experiments in neurons with widefield imaging of calcium signaling (BAPTA-1) and STED timelapse imaging of actin (SiR-actin). | Nature Methods

Extended Data Fig. 3: etSTED experiments in neurons with widefield imaging of calcium signaling (BAPTA-1) and STED timelapse imaging of actin (SiR-actin).

From: Event-triggered STED imaging

Extended Data Fig. 3

a, Mean widefield image of all triggering widefield frames. b, Maximum-projected analysis ratiometric image of all detected events in experiment. Green squares show location of detected events (a,b). c, Zoomed-in views of the ratiometric image in two detected events. d, 0.99 Hz etSTED timelapse of the actin structures. N = 234 events, N = 19 cells. Overlaid semi-transparent white outlines show extent of detected local calcium activity. Scale bars, 10 µm (a,b), 3 µm (c), 1 µm (d).

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