Fig. 3: In vivo real-time measurement of brain oxytocin oscillation in freely behaving mice.
From: A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain
a, Schematic illustrating fiber photometry recording of MTRIAOT in the AON in freely behaving mice. b, Representative traces of 470-nm-excited signals (green) and 405-nm-excited signals (blue) from either MTRIAOT-expressing or MTRIAOT-mut-expressing mice. c, Summary of peak z-scores (n = 4 mice). Statistics used were one-way ANOVA (F3,12 = 3.49, P = 7.4 × 10−5) with Bonferroni post hoc test (P = 0.026, 470 nm versus 405 nm in MTRIAOT; P = 0.034, 470 nm in MTRIAOT versus 470 nm in MTRIAOT-mut; P = 0.21, 470 nm versus 405 nm in MTRIAOT-mut). d, Frequency histogram showing the intervals of OT signal peaks (n = 26 events from four mice). e, Schematic illustrating experimental protocol for assessing the involvement of OT release from PVN neurons in OT signal increases in the AON. f, Representative traces of MTRIAOT activities recorded from mice either expressing mSca or coexpressing TeLC and mSca in the PVN. g,h, Summary of the peak number every hour (g) and peak z-score (h; n = 3 mice in mSca and n = 4 mice in TeLC-P2A-mSca). Statistics used were an unpaired two-tailed t-test (P = 8.2 × 10−4 in g and P = 1.6 × 10−4 in h). Graphs represent the mean ± s.e.m. (c, g and h). ***P < 0.001, *P < 0.05, NS (c, g and h).
