Fig. 4: In vivo real-time measurement of brain oxytocin responses upon social interaction and acute stress.
From: A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain
a, Schematic illustrating fiber photometry recording of social interaction-induced OT responses. b, Mean z-scored traces (green thick lines) showing MTRIAOT responses to encounters with either a mouse (top) or a toy (bottom). The regions covering s.d. values of the traces are shaded in light green. Stimulation was applied during the periods shaded in pink. c, Summary of AUC values during stimulation (n = 6 mice). Statistics used were a paired two-tailed t-test (P = 0.03). d, Summary of rise time constants for social interaction-induced OT responses determined by a single-exponential fitting (n = 6 mice). e, Histological verification of sensor expression around the recording region (left) and schematic illustrating fiber photometry recording of social interaction-induced OT responses (right). f, Mean z-scored traces showing MTRIAOT responses (green, 470-nm-excited signal; blue, 405-nm-excited signal) either following tail lift (left, tail lift) or without simulation (right: no stim.). The regions covering s.d. values of the traces are shaded in light colors. Tail lift was applied during the periods shaded pink. g, Summary of AUC values during stimulation (n = 18 trials from three mice). Statistics used were one-way ANOVA (F3,68 = 2.74, P = 7.5 × 10−14) with Bonferroni post hoc test (P = 5.0 × 10−5, 470 versus 405 in tail lift, P = 1 in 470 versus 405 in no stim.). h, Summary of times to peak for tail lift-induced OT responses (n = 18 trials from three mice). Scale bar, 200 µm (e). Graphs represent the mean ± s.e.m. (c and g) and mean ± s.d. (b, d, f and h). ***P < 0.001, *P < 0.05, NS (c and g).
