Fig. 5: Alterations in brain oxytocin levels caused by anesthesia, food deprivation and aging.
From: A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain
a, Schematic illustrating the recording and representative trace of MTRIAOT activity showing the impact of mix-anes on OT oscillation. The background of the trace is shaded to indicate the period of anesthesia after mix-anes administration (dark blue) and period after release by the antagonist, atipamezole (pink). b, Summary of minimum z-score values before mix-anes administration, after administration of mix-anes and following subsequent administration of atipamezole (n = 4 mice in before and mix-anes, n = 3 mice in atipamezole). c, Schematic of the recording and representative trace of MTRIAOT activity showing the impact of isoflurane on OT signal. The background of the trace is shaded to indicate the period of 1% isoflurane administration (gray), of 4% isoflurane administration (dark blue), and after release from the anesthesia (0%, pink). d, Summary of the tail values of z-scores during the above three states (n = 5 mice). e, Representative trace of MTRIAOT activity showing the impact of food deprivation on OT oscillation. The background of the trace is shaded to indicate the period of food deprivation. The period of OT turbulence is colored dark blue and the peaks of undershot signal are indicated by arrowheads. f, Summary of minimum z-score values before, during and after food deprivation (n = 3 mice). g, Representative traces of MTRIAOT fluorescence signals in mice at the indicated age (2 months, 6 months, 1 year or 2.5 years). h, Summary of the peak number every hour (top) and peak z-score (bottom; n = 3 mice). Graphs represent the mean ± s.e.m. (b, d, f and h). ***P < 0.001, *P < 0.05 and NS unless otherwise stated (b, d, f and h). Statistics (b, d, f and h) are summarized in Supplementary Note 3.
