Extended Data Fig. 4: ISSAAC-seq experimental results from different cell lines and PBMCs.

a, Examples of a typical FACS based workflow using K562 cells. FSC-A and SSC-A were used to select intact nuclei (P1). FSC-A and FSC-W were used to select singlets (P2). DAPI-A was used to select DAPI positive nuclei (P3). b, DNA electrophoresis of typical ISSAAC-seq libraries from the FACS (left) and droplet (right) workflow using K562 cells. c and d, Fragment size (c) and Tn5 insertion frequency (d) of ATAC data using different methods in the indicated cell lines. e, Quality metrics of the ISSAAC-seq PBMC data. f, Genomic distribution of reads from RNA data using different methods. g, Distributions of the numbers of UMIs using different methods performed in the indicated cell lines and PBMCs. Boxplots show median with the top and bottom edges of the box representing the 75th and 25th percentiles, respectively. The whiskers represent the most extreme values that are within 1.5 times the interquartile range of the 25th and 75th percentiles. Outliers outside the whiskers are not shown. The exact number of cells can be found in the Source Data.