Extended Data Fig. 6: H3K27me3 RT&Tag performance with decreasing number of nuclei input.
From: Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation
a) Heatmap showing the average IgG and H3K27me3 RT&Tag signal from two experiments performed using either 100,000 or 25,000 nuclei. Individual rows represent the 1342 transcripts identified as H3K27me3-enriched in Fig. 3b. The heatmap colors represent z-score scaling across rows. b) Genome browser tracks showing the distribution of IgG and H3K27me3 RT&Tag signal from 100,000, 25,000, or 5000 nuclei over the gene bodies of CR43334 and CR42862. Combined reads from 2 replicates are shown. c) Boxplots showing the IgG and H3K27me3 RT&Tag signal (Counts per million, CPM) from 100,000, 25,000, or 5000 nuclei for the HOX cluster genes. *FDR < 0.05, n = 2 independent RT&Tag experiments.
