Extended Data Fig. 7: RT&Tag captures transcripts within polycomb domains.
From: Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation
a) Dot plot showing the top 10 GO biological process terms associated with H3K27me3-enriched transcripts. The dot size corresponds to the gene count and the color represents statistical significance (hypergeometric test, benjamini hochberg p value adjustment). b) Profile plot showing the H3K27me3 CUT&Tag signal (combined reads from 2 replicates) over the gene bodies of the top 25% expressed genes. Error bars indicated the standard error. c) Boxplot showing the RNA-seq expression levels (Counts per million, CPM) of H3K27me3-RT&Tag enriched transcripts that had either high (>9 read counts) or low (<9 read counts) H3K27me3 CUT&Tag signal over their gene bodies. *p = 0.00488, Welch Two Sample t-test (two-sided), n = 1133 for high H3K27me3, n = 207 for low H3K27me3, n = 2 for RNA-seq. For the boxplots, the IQR is shown within the limits of the box, the center line represents the median, the whiskers show data that is within 1.5 times the IQR, and outliers are omitted.
