Fig. 2: RT&Tag captures the interaction between MSL2 and roX2.
From: Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation
a, Illustration showing RT&Tag being used to capture the interaction between MSL2 and roX2. b, Tapestation gel image and corresponding electropherogram showing size distribution of the MSL2 RT&Tag libraries after two rounds of 0.8× bead cleanup. This image is representative of two independent experiments. FU (fluorescence units). c, Pie chart showing the proportion of MSL2 RT&Tag reads (n = 4) aligning to regions classified as exonic, intronic or intergenic. d, Density plot showing the distribution of aligned MSL2 RT&Tag reads (n = 4) scaled over Drosophila gene bodies. e, PCA showing separation between IgG and MSL2 RT&Tag samples (n = 4) along the first principal component (PC1) and separation between replicates in the second principal component (PC2). The first two and last two replicates have been sequenced on two separate flow cells and hence a batch effect may be observed. f, Volcano plot showing transcripts differentially enriched for MSL2 over IgG RT&Tag (FC >2, FDR < 0.05, n = 4). Transcripts enriched for MSL2 are highlighted in red, nonenriched are in black and depleted are in blue. g, Genome browser track showing the distribution of MSL2 and IgG RT&Tag signal over the gene body of roX2. Combined reads from four replicates are shown. h, Karyoplots showing the bins (50 bp) where MSL2 RT&Tag signal is fourfold over IgG plotted (n = 4) over the Drosophila chromosomes. i, Profile plots showing the MSL2 (top) and H4K16ac (bottom) CUT&Tag signal around the TSS (top) and gene bodies (bottom) of MSL2 RT&Tag-enriched or nonenriched transcripts. Combined reads from two replicates for MSL2 CUT&Tag and one replicate for H4K16ac CUT&Tag are shown. Error bands indicate standard error.
