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Advances in nanopore direct RNA sequencing

Nature Methods volume 19, pages 1160–1164 (2022)Cite this article

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Nanopore direct RNA sequencing (DRS) reads continuous native RNA strands. Early adopters have used this technology to document nucleotide modifications and 3′ polyadenosine tails on RNA strands without added chemistry steps. Individual strands ranging in length from 70 to 26,000 nucleotides have been sequenced. In our opinion, broader acceptance of nanopore DRS by molecular biologists and cell biologists will be accelerated by higher basecall accuracy and lower RNA input requirements.

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Fig. 1: Nanopore sequencing of individual E. coli 16S ribosomal RNA strands.
Fig. 2: General schemes for modification detection using nanopore DRS.

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Acknowledgements

M.J., H.E.O. and R.A.S. were supported by NIH grant HG010053.

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Authors and Affiliations

  1. Northeastern University, Boston, MA, USA

    Miten Jain

  2. University of California, Santa Cruz, CA, USA

    Robin Abu-Shumays, Hugh E. Olsen & Mark Akeson

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  1. Miten Jain
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  2. Robin Abu-Shumays
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Correspondence to Miten Jain or Mark Akeson.

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Competing interests

M.A. holds options in ONT and is a paid consultant to ONT. H.E.O. and M.J. received reimbursement for travel, accommodation and conference fees to speak at events organized by ONT.

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Jain, M., Abu-Shumays, R., Olsen, H.E. et al. Advances in nanopore direct RNA sequencing. Nat Methods 19, 1160–1164 (2022). https://doi.org/10.1038/s41592-022-01633-w

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