Extended Data Fig. 9: Additional electrophysiological characterizations of cAMPFIREs in CA1 neurons. | Nature Methods

Extended Data Fig. 9: Additional electrophysiological characterizations of cAMPFIREs in CA1 neurons.

From: Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo

Extended Data Fig. 9

a & b, Quantification of the elicited AMPAR and NMDAR currents (in pA) of CA1 neurons transfected with cAMPFIRE-L or cAMPFIRE-M compared to adjacent untransfected control neurons. For both, n = 10 neuronal pairs for cAMPFIRE-L and 16 for cAMPFIRE-M. Blue points and error bars indicate mean and s.e.m., respectively. c & d, example traces (c), and amplitudes (d) of mEPSCs (n = 9 for -L and 7 for -M) of CA1 neurons transfected with cAMPFIRE-L and cAMPFIRE-M compared to adjacent untransfected control neurons. e, Quantification of the paired pulsed ratio (n = 11 for -L and 15 for -M) of CA1 neurons transfected with cAMPFIRE-L and cAMPFIRE-M compared to adjacent untransfected control neurons. n.s.: not significant, paired two-tailed Student’s t-test. Colored points and error bars indicate mean and s.e.m., respectively. For panels d and e from left to right, P = 0.55, 0.87, 0.12, and 0.42, dF = 8, 6, 10 and 14.

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