Extended Data Fig. 10: cAMPFIREs detect cAMP responses in Drosophila larvae. | Nature Methods

Extended Data Fig. 10: cAMPFIREs detect cAMP responses in Drosophila larvae.

From: Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo

Extended Data Fig. 10

a, Schematic showing experimental setup. Larvae were dissected to expose the intact central nervous system (boxed region) and imaged with a water immersion objective. b & c, Average traces (b) and collective responses measured from the gray shaded area in panel b (c) of abdominal leucokinin neuronal somas expressing cAMPFIRE-M following application of forskolin (40 µM) or DMSO-only control. In panel b, Dark lines indicate mean and shaded areas indicate s.e.m. Arrowhead indicates time of bath application. In panel c, bars indicate mean. ***: P = 1.1 × 10−5, two-tailed Wilcoxon Rank-Sum test. n = 10 larvae per condition. d & e, Average traces (d) and collective responses measured from gray shaded area in panel d (e) of fly larval nociceptive axon terminals in response to bath application of AITC (allyl isothiocyanate; 2.5 mM) followed by forskolin (40 µM) and IBMX (100 µM). AITC is a stimulant for larval nociceptors. In panel d, dark lines indicate mean and shaded areas indicate s.e.m. In panel e, bars indicate mean. n.s.: not significant (p = 0.49), *: P = 0.049, **: P = 0.0048, two-tailed one-way ANOVA with Tukey’s multiple comparisons. n = 6 larvae per condition.

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