Extended Data Fig. 1: Correcting the perinuclear localization of the Epac-SH187 sensor.
From: Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo

a, Crystal structure of the NPL sequence in Epac2A (top) and the alignment of NPL sequences between Epac1 with Epac2. The positions tested are highlighted in the structure (red) and at the alignment (asterisks). b, Representative images of the indicated mutants compared to Epac-SH187 in HEK cells. From left to right starting from the top, n (cells/coverslips) = 69/29, 50/5, 59/29, 18/3, 12/2, 12/2, 37/4. c, Response time course (left) and quantification of the response of indicated constructs to the indicated stimuli in HEK cells. From left to right, n = 20, 13, and 11. Dark lines and bars indicate mean. Shaded area indicates s.e.m. d, Representative images of the indicated mutant compared to Epac-SH187 in L2/3 pyramidal neurons. Single two-photon optical sections are shown. n (cells/mice) = 18/5 and 20/8 for Epac-SH187 and L777A/K778A, respectively.