Extended Data Fig. 2: Localization-correcting mutations reduce the tendency of the sensor to aggregate.
From: Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo

a & b, Representative images (a) and quantifications (b) of the number of aggregates per cell resulting from the indicated constructs. To ease the visualization of aggregates, saturated pixels (for display only) are showed in red. Single cpV: a sensor variant that is the same as Epac-SH187 with the exception that it only has a single cpVenus as the FRET acceptor. From left to right, n (cells) = 12, 58, 53, 48, and 41. Lines indicate mean. ***: P < 0.001 (from left to right, P = 3.3 × 10−10, 5.2 × 10−10, and 1.7 × 10−10) for all comparisons, two-tailed Welch test.