Extended Data Table 1 Biochemical characterization of selected FPs and their brightness in human cells. Biochemical characterization of selected FPs and their brightness in human cells. Note: except for moxGFP, eGFP-C48S/C70V, and Clover-C48S/C70V, data above the ‘FOLD4’ row are cited from Campbell et al., PNAS, 2020, for the purpose of structure-activity comparison. All data in the table were generated in our lab in this study unless otherwise stated. ϕ= quantum yield; ε = extinction coefficient at peak absorbance. N.D.: not determined. aMolecular brightness = (ϕ × ε)/103. bmoxGFP is human codon-optimized sfGFP-C48S/C70V; we have re-characterized it for this study. cIn BE(2)-M17 cells. See Supplementary Fig. 5g for results from additional human cell lines. dThe quantum yield of moxGFP is cited from Costantini et al., Nat. Comm., 2015. All other data in the table were generated in our lab. eMolecular brightness of moxGFP was determined using our EC value and the cited QY value. Mean ± : s.d., n ≥ 3 experiments; n = 2 experiments for Tm and λex/λem values
From: Chemically stable fluorescent proteins for advanced microscopy
