Extended Data Fig. 5: Additional example 1 of VSV-G VLP freely diffusing in the extracellular space.
From: Capturing the start point of the virus–cell interaction with high-speed 3D single-virus tracking
a, Top-down view of virus trajectory pincering live HeLa cell. b, Virus-to-cell distance representation of trajectory in a. a and b share the same axes and scale bar. c,d, Different view of the trajectory shown in a, b. Circular insets shows close-up views of virus in close contact with the cell surface. c and d share the same axes and scale bar. e, Cross-section with slice along x and y axis, overlaid with trajectory color-coded by diffusion coefficient. a, c, and e are color-coded by image intensity, while b and d display virus-to-cell distance map on surface of cell. f, Top, correlation between diffusivity and distance from the cell surface of VSV-G VLP, close-approaches (below 0.5 µm) are color patched purple. Dashed purple line, 0.5 µm from cell surface. Below left, enlarged view of data between 14.5-25.5 sec, associated with trajectory segment displayed in circular inset of d. Below right, enlarged view of data between 43.25-56 sec, associated with trajectory segment displayed in circular inset of d. g, Visual representation of virus-to-cell distance calculation. Isosurface volume render of cell image (gray) overlayed with trajectory sampled at 3 msec (white). Distance vectors are calculated from each trajectory timepoint to the isosurface (generated by top-hat transform) and displayed as color-coded spheres on the cell surface.
