Extended Data Fig. 7: Additional example 1 of VLP-protrusion interaction.
From: Capturing the start point of the virus–cell interaction with high-speed 3D single-virus tracking
a, Top-down view of VSV-G VLP landing on protrusion from the surface of a live cell. 3D volume rendering of live HeLa cells (stained with SYTO61) co-registered with high resolution virus trajectory from 4D tracking and imaging data. b, c Alternate views of the trajectory shown in a. d, Magnified views showing cylindrical nature of trajectory and proximity to cell surface. e, Top-down view, with trajectory colored by diffusion coefficient, free portion with D > 1.5 × 10−2 μm2/s colored gray. f, Color-coded trajectory, showing free diffusion (D > 1.5 × 10−2 μm2/s, gray) and bound diffusion (green-yellow). g, Cylindrical fitting of the bound portion of the trajectory in a-f (28-120 sec), fitted cylinder (magenta) has a radius of 122 ± 8.4 nm after taking into consideration the tracked particle diameter. h, View of trajectory along the cylinder axis. i, Intensity trace of tracked VLP.
