Extended Data Fig. 8: High resolution tracking and simultaneous 3D imaging localizes VSV-G VLP diffusion along fibroblast protrusion.
From: Capturing the start point of the virus–cell interaction with high-speed 3D single-virus tracking
a, Left, top-down view of VSV-G VLP ‘surfing’ on actin-stained protrusion of BJ fibroblast. Trajectory colored by time. The lower, brighter, z-planes have been omitted to help visualize the protrusion the VLP is localized to. Right, same view but trajectory color-coded by diffusivity. b, Snapshots of 3D reconstruction. The sustained linear motion correlates with the lateral surface of the protrusion, large changes in diffusivity where detected. c, (left) XY MIP, (middle) magnified, and (right) YZ MIP of viral diffusion on actin-rich protrusion.
