Extended Data Fig. 10: Multi-trajectory acquisition.
From: Capturing the start point of the virus–cell interaction with high-speed 3D single-virus tracking
a, Left: 10 VSV-G VLP trajectories collected in the same area of live HeLa cells stained with SYTO61 (see also: Supplementary Fig. 29 and Supplementary Video 8). Right: top-down (xy) MIP with multiple co-registered VLP trajectories. Colorbar represents trajectory number, with each trajectory uniquely colored. b, The dwell time of 27 VSV-G VLP trajectories (starting from free diffusion) collected in the same area as a were binned into voxels and represented as spheres, color-mapped by diffusivity. This correlated analysis reveals commonly visited regions and highlights the slower diffusion experienced by the VLP population nearer the cell surface. Inset: two different VLPs land in close proximity on the same cell. c, Exploded views showing multiple VLPs contact the same cell.
