Extended Data Fig. 1: Instrument Diagram.
From: Capturing the start point of the virus–cell interaction with high-speed 3D single-virus tracking
3D-TrIm Instrument Diagram. The 3D-TrIm microscope consists of two excitation sources: one for single-particle tracking and one for imaging. The tracking laser position is modulated using two electro-optic deflectors (EOD) and tunable acoustic gradient (TAG) lens. The imaging laser focus is modulated by an electrically tunable lens (ETL) and the beam is sampled by beam-splitters (BS) and evaluated using a system of photodiodes (PD). This focus is relayed using two lenses (IL). The tracking laser enters the underside of the LSM410 through a dichroic mirror (DCM2) where it ultimately couples with the imaging laser through DCM1 toward the shared objective lens and piezoelectric stage. After exciting the sample, the fluorescence emission pathway is shared between tracking and imaging until separated by DCM3.
