Extended Data Fig. 6: Image-seq of stromal cells.
From: Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging
a) Example of the gating strategy used to isolate CXCl12+, CD45− stromal cells from a micropipette sample for cell lysis and SMARTseq-v4 library preparation. Sorted cells were gated on singlet live cells (DAPI-). b) UMAP embedding of the stroma SMARTseq-v4 data using the top 30 principal components, color coded by cell population. c) Heatmap of scaled normalized expression for the 20 most highly expressed genes within each cell cluster. d) Heatmap of scaled normalized expression for Lepr-MSC and Endothelial cell marker genes from the literature27. UMAP: uniform manifold approximation and projection. MSC: Mesenchymal stromal cells, EC: endothelial cells.
