Extended Data Fig. 7: Cell cycle analysis of AML cells from Fig. 4, along with selected intravital microscopy data.
From: Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging
a) Percentage of AML cells that are CellTracker Red+ on day 3 (CellTracker + (%)) as a function of the number of cells per cavity. b) Violin plots of selected cell cycle relevant genes from Supplementary Table 4. Statistical significance was determined using a two-sided Student’s t-test (Sample size: NP = 33, IM = 17, P = 34 cells, 11 independent mice). c) Heatmap showing scaled average expression of gene sets in distinct phase of cell cycle, each column indicates a cell. AML cells are clustered into three groups by hierarchical clustering based on cell cycle genes. d) Representative images of P and NP cells from mice injected with rat IgG2a,κ-AF568 antibody (DPP4 isotype control). Imaging of DPP4 isotype control labeling was performed in 14 biological replicates with NP cells and 6 biological replicates with P cells (1 independent mouse). Definition of P/ NP/IM based on cell number at day 3.
