Extended Data Fig. 10: Detection of DPP4 on in vitro cultured cells.
From: Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging
a) Cell surface antibody labeling with DPP4 in HA9M1, analyzed by flow cytometry. b) Analysis of Dpp4 expression by QPCR on in vitro cultured HA9M1 and MLL-AF9 cells. Results are compared to splenic T cells, which express reproducible levels of Dpp4. (N = 3 biological replicates from 2 independent experiments). Error bars denote standard deviation. c) Flow cytometry analysis of DPP4 expression in HA9M1 by intracellular staining with DPP4 antibody. d) Analysis of Dpp4 by QPCR on AML cells collected from the bone marrow 3 weeks after transplantation. DPP4+ and DPP4− HA9M1 cells were sorted by flow cytometry and their gene expression compared to that of splenic T cells by Q-PCR. After initial analysis, the T cells and DPP4+ /− HA9M1 cells were cultured for 4 days using the same media and analyzed once again for Dpp4 expression. HA9M1: Hoxa9/Meis1-Ubiquitin-c-GFP (N = 3 biological replicates from 2 independent experiments). Statistical significance was calculated by one-way ANOVA followed by Sidak’s post-hoc test, error bars denote standard deviation.
