Fig. 6: The leukemia microenvironment.
From: Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging
a, DPP4 expression on AML cells, analyzed by cell surface staining and flow cytometry, after 3 day co-culture with stromal cells. BM stroma, immortalized bone marrow stroma from D.B.S.’s laboratory; MC_3T3_E1, osteoblast precursor cell line; MLO_A5, murine long bone osteocyte cell line; MS-5, mouse stroma cell line; NIH-3T3, fibroblast cell line. (Control, NIH-3T3, MS-5 and BM stroma, n = 6; MC_3T3 and MLO_A5, n = 3 biological replicates; two independent experiments). Statistical significance was calculated using one-way ANOVA followed by Šídák’s multiple comparisons test. Error bars represent the standard deviation and are centered at the arithmetic mean. b, Distribution of HA9M1 cells in D, M and R cavities on day 0, 1 and 3 after transplantation. Day 0: D0, n = 22 cavities, M0 n = 82 cavities, R0 n = 41 cavities (6 independent mice); day 1: D1 n = 21, M1 n = 84, R1 n = 43 cavities (6 independent mice); day 3: D3 n = 16, M3 n = 95, R3 n = 21 cavities (7 independent mice). Error bars represent the standard deviation and are centered at the arithmetic mean. Statistical significance was determined using an unpaired, two-sided Mann–Whitney U-test. The 99.9% confidence intervals for P, IM and NP cells from Fig. 4b,c are marked by red, blue and green rectangles, respectively. HA9M1, Hoxa9/Meis1-Ubiquitin-c-GFP; IsoT, isotype; MFI, mean fluorescence intensity.
