Extended Data Fig. 3: Long bone cell extraction.
From: Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging
a) 3D visualization of a target location in a β-actin-GFP mouse before and after cell aspiration. The bottom panel is obtained by rotating the top panel by 180° around the x axis. The isolated cell volume is marked by an orange outline. b) Comparison of micropipette and whole bone marrow preparations from the calvarium and tibia. Statistical analysis was performed using the two-way ANOVA for each individual cell population, with the two independent variables being bone type (tibia vs calvarium) and procedure (micropipette vs whole bone marrow preparations). Listed p values were not adjusted for multiple comparisons. Using a Bonferroni correction, statistical significance is achieved for p < 0.006 (𝛂=0.05); significant p values are highlighted in red.
