Fig. 4: Spatial multi-omics in murine spleen enabled by MARIO matching.
From: Robust single-cell matching and multimodal analysis using shared and distinct features
a, t-SNE plots of murine spleen CITE-seq and CODEX cells, preintegration and MARIO integration, colored by the dataset of origin (left and middle) or colored by cell-type annotation (right). b, A murine spleen section colored by the cell-type annotation from CODEX (top left) or the label transferred annotation from CITE-seq (middle left). Examples of RNA transcripts ((Il7r, Ms4a1, Cxcr5 and Myc) and their tissue-specific localization are inferred through MARIO integrative analysis (middle and right columns). An enlarged view of the tissue region demarcated by the orange box is shown in the bottom row. Scale bars, 400 μm (upper) and 200 μm (lower). Dotted line, T cell zone; white line, B cell zone. c, Heatmap of differentially expressed genes (from matched CITE-seq cells) among subpopulations of CODEX B cells, gated based on CODEX proteins. Four subpopulations of B cells were identified: transitional type 1 B cells (T1), marginal zone B cells (MZ), mature B cells (M), and follicular/germinal center B cells (FO/GC).
