Extended Data Fig. 9: Photostability of QuasAr6a and QuasAr6b in vivo (Related to Fig. 4).
From: Video-based pooled screening yields improved far-red genetically encoded voltage indicators
a. Raw Arch-channel fluorescence trace without baseline or photobleaching correction of a Layer 1 NDNF cell (visual cortex) expressing QuasAr6a-based Optopatch, imaged for 200 seconds at 1 kHz (n = 2 cells). The 635-nm power delivered to the cell was 4 mW. b. Raw Arch-channel fluorescence trace of a hippocampal PV cell expressing QuasAr6b-based Optopatch, imaged for 200 seconds at 2 kHz (n = 2 cells). The 635-nm power delivered to the cell was 8 mW. The measurement was done in anesthetized animals. The fluorescence traces were the raw traces directly extracted from cell mask and not corrected for background. The SNR and FWHM was calculated for the all the optogenetically evoked spikes in the magnified region.
