Extended Data Fig. 3: Engineering QuasAr6a and QuasAr6b (Related to Fig. 2).
From: Video-based pooled screening yields improved far-red genetically encoded voltage indicators
a. Pipeline for engineering improved GEVIs. b. Comparison of the previously reported mutations (orange),26,34 and newly identified mutations in this study (lime-green, pale cyan and blue assigned in accordance with Fig. 3a). c. Violin plot for the per-molecule brightness (FArch/FCitrine) of single mutants expressed in HEK cells. The per-molecule brightness was normalized by the average per-molecule brightness of Archon1-Citrine in HEK cells. The residues selected for engineering QuasAr6a/b are shown in bold. d. Violin plot for the expression level (FCitrine) of single mutants expressed in in HEK cells. The values were normalized to the average expression level of Archon1-Citrine in HEK cells.
